Transposon mutagenesis in Desulfovibrio desulfuricans: development of a random mutagenesis tool from Tn7.
AUTOR(ES)
Wall, J D
RESUMO
The transposons Tn5, Tn7, Tn9, and Tn10 or their derivatives have been examined for transposition in the sulfate-reducing bacterium Desulfovibrio desulfuricans G20. Tn7 inserted with a frequency of 10(-4) to 10(-3) into a unique attachment site that shows strong homology with those sites identified in other gram-negative bacteria. Inactivation of the tnsD gene in Tn7, encoding the function directing insertion into the unique site, yielded a derivative that transposed essentially randomly with a frequency of ca. 10(-6) per donor. Derivatives of Tn5, but not wild-type Tn5, were also found to undergo random transposition at a similar frequency. No evidence was obtained for transposition of Tn9 or Tn10.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=168183Documentos Relacionados
- Identification of a transposon Tn7-dependent DNA-binding activity that recognizes the ends of Tn7.
- Site-specific insertion of three structural gene cassettes in transposon Tn7.
- Reduction of Technetium by Desulfovibrio desulfuricans: Biocatalyst Characterization and Use in a Flowthrough Bioreactor
- Characterization of trimethoprim resistance by use of probes specific for transposon Tn7.
- Tn7-Based Genome-Wide Random Insertional Mutagenesis of Candida glabrata