Trichloroethylene removal and oxidation toxicity mediated by toluene dioxygenase of Pseudomonas putida.
AUTOR(ES)
Heald, S
RESUMO
Whole cells of Pseudomonas putida containing toluene dioxygenase were able to remove all detectable trichloroethylene (TCE) from assay mixtures. The capacity of cells to remove TCE was 77 microM/mg of protein with an initial rate of removal of 5.2 nmol/min/ng of protein. TCE oxidation resulted in a decrease in the growth rate of cultures and caused rapid cell death. Addition of dithiothreitol to assay mixtures increased the TCE removal capacity of cells by up to 67% but did not prevent TCE-mediated cell death. TCE induced toluene degradation by whole cells to a rate approximately 40% of that induced by toluene itself.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=202037Documentos Relacionados
- Toxicity of Trichloroethylene to Pseudomonas putida F1 Is Mediated by Toluene Dioxygenase
- Degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with Pseudomonas putida F1.
- Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes.
- Catabolite-mediated mutations in alternate toluene degradative pathways in Pseudomonas putida.
- Cyclodextrin-enhanced degradation of toluene and p-toluic acid by Pseudomonas putida.