Triterpenes from the Protium heptaphyllum resin - chemical composition and cytotoxicity

AUTOR(ES)

Lima, Ewelyne M., Nascimento, Andrews M., Lenz, Dominik, Scherer, Rodrigo, Meyrelles, Silvana S., Boëchat, Giovanna A.P., Andrade, Tadeu U., Endringer, Denise C.

FONTE

Rev. bras. farmacogn.

DATA DE PUBLICAÇÃO

2014-08

RESUMO

Protium heptaphyllum (Aubl) Marchand, Burseraceae, is popularly used as an analgesic and anti-inflammatory agent. However, the cellular mechanism of action remains unknown. This study aims to evaluate the chemical composition of P. heptaphyllum resin and cytotoxicity on a breast cancer cell line (MCF-7). The chemical composition of the resin was determined by Gas Chromatography coupled to a Mass Spectrometer. The cytotoxicity was evaluated using an MTT assay. Annexin V-FITC, caspase-3, Angiotensin Converting Enzyme activity and Tumor Necrosis Factor alpha (TNF- α) assays were performed to evaluate apoptosis and inflammatory events. The resin consisted of triterpenes, such as α- and β-amyrin. Cytotoxicity was only observed in fractions enriched with α- and β-amyrin. The resin and fractions elicited antiproliferative activity, increased activity of caspase-3 and ACE, and a decrease in the TNF-α level. Altogether, the resin and fractions enriched with α- and β-amyrin promoted cytotoxicity and apoptosis.

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