Turnover of primary transcripts is a major step in the regulation of mouse H19 gene expression
AUTOR(ES)
Milligan, Laura
FONTE
Oxford University Press
RESUMO
In the gene expression pathway, RNA biogenesis is a central multi-step process where both message fidelity and steady-state levels of the mature RNA have to be ascertained. An emerging question is whether RNA levels could be regulated at the precursor stage. Until recently, because it was technically very difficult to determine the level of a pre-mRNA, discrimination between changes in transcriptional activity and in pre-mRNA metabolism was extremely difficult. H19 RNA, the untranslated product of an imprinted gene, undergoes post-transcriptional regulation. Here, using a quantitative real-time RT–PCR approach, we accurately quantify its precursor RNA levels and compare these with the transcriptional activity of the gene, assessed by run-on assays. We find that the levels of H19 precursor RNA are regulated during physiological processes and this regulation appears to be related to RNA polymerase II transcription termination. Our results provide direct evidence that turnover of polymerase II primary transcripts can regulate gene expression in mammals.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1084202Documentos Relacionados
- Two regulatory domains flank the mouse H19 gene.
- The structural H19 gene is required for transgene imprinting
- H19 and Igf2 monoallelic expression is regulated in two distinct ways by a shared cis acting regulatory region upstream of H19
- The product of the imprinted H19 gene is an oncofetal RNA.
- Deletion of the H19 differentially methylated domain results in loss of imprinted expression of H19 and Igf2