Two classes of proteins and mRNAs in Lilium longiflorum L. indentified by human vitronectin probes.
AUTOR(ES)
Wang, C S
RESUMO
Vitronectin (VN) is a substrate adhesion molecule, an extracellular matrix glycoprotein that facilitates cell adhesion and cell movement in animals. We have reported the cross-reactivity of a 55-kD protein in plants with rabbit anti-human VN antibodies and the presence of VN-like sequences in plant genomes using a human VN cDNA probe. We have extended these studies by using human VN riboprobes to detect VN-like mRNAs in lily (Lilium longiflorum L.) and soybean. In both species, two mRNAs were detected. We have also identified a new cross-reactive protein (41 kD) using a different preparation of human VN antiserum. In lily roots five 41-kD isoforms were observed, whereas only three of these isoforms accumulated in leaves. Monospecific antibodies prepared against the plant proteins cross-reacted with the human VN protein and vice versa. We have purified the 41-kD protein using two-dimensional gel electrophoresis, and amino acid composition analysis indicates that it is similar in composition to human VN.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=159250Documentos Relacionados
- Developmental regulation and phytochrome-mediated induction of mRNAs encoding a proline-rich protein, glycine-rich proteins, and hydroxyproline-rich glycoproteins in Phaseolus vulgaris L.
- Two preproendothelin 1 mRNAs transcribed by alternative promoters.
- Localization of mRNAs and Proteins in Methyl Methacrylate–embedded Tissues
- Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies.
- Human serum amyloid A. Three hepatic mRNAs and the corresponding proteins in one person.
