Two distinct effects on neurotransmission in a temperature-sensitive SNAP-25 mutant

AUTOR(ES)

Rao, Sujata S.

FONTE

Oxford University Press

RESUMO

Vesicle fusion in eukaryotic cells is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors). In neurons, the t-SNARE SNAP-25 is essential for synaptic vesicle fusion but its exact role in this process is unknown. We have isolated a SNAP-25 temperature-sensitive paralytic mutant in Drosophila, SNAP-25ts. The mutation causes a Gly50 to Glu change in SNAP-25’s first amphipathic helix. A similar mutation in the yeast homologue SEC9 also results in temperature sensitivity, implying a conserved role for this domain in secretion. In vitro-generated 70 kDa SNARE complexes containing SNAP-25ts are thermally stable but the mutant SNARE multimers (of ∼120 kDa) rapidly dissociate at 37°C. The SNAP-25ts mutant has two effects on neurotransmitter release depending upon temperature. At 22°C, evoked release of neurotransmitter in SNAP-25ts larvae is greatly increased, and at 37°C, the release of neurotransmitter is reduced as compared with controls. Our data suggest that at 22°C the mutation causes the SNARE complex to be more fusion competent but, at 37°C the same mutation leads to SNARE multimer instability and fusion incompetence.

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