Two distinct intervening sequences in different ribosomal DNA repeat units of Sciara coprophila.
AUTOR(ES)
Renkawitz-Pohl, R
RESUMO
We have prepared a partial gene library of sheared DNA from the fungus fly, Sciara coprophila, by dA-T tailing and insertion into pBR322. Two ribosomal DNA clones which differ from the usual ribosomal DNA organization in this organism were studied in detail. Clone pBc 1L-1 has an intervening sequence of 1.4 kb, and clone pBc 6D-6 has an intervening sequence of 0.9 kb. These intervening sequences occur in about the same position in 28S rDNA, but do not appear to share sequence homology with one another. Previously we found that 90% of Sciara ribosomal DNA is homogenous and lacks an intervening sequence, and our present data explains the size heterogeneity found in most of the remaining 10%. We have found no evidence of size heterogeneity in the nontranscribed spacer.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327389Documentos Relacionados
- The role of ecdysone in DNA-puff formation and DNA synthesis in the polytene chromosomes of Sciara coprophila.
- Coding and spacer sequences in the 5.8S-2S region of Sciara coprophila ribosomal DNA.
- Further Studies on the Ribosomal RNA Cistrons of SCIARA COPROPHILA (Diptera)
- rRNA processing: removal of only nineteen bases at the gap between 28S alpha and 28S beta rRNAs in Sciara coprophila.
- Sex Determination in a "Bisexual" Strain of Sciara Coprophila