Two nuclear mutations that block mitochondrial protein import in yeast.
AUTOR(ES)
Yaffe, M P
RESUMO
We isolated two yeast mutants that are temperature-sensitive for import of mitochondrial proteins. Each strain contains a single mutation that results in arrest of growth and accumulation of precursor to the beta subunit of the mitochondrial F1-ATPase after incubation at 37 degrees C. These lesions (mas1 and mas2) are nonallelic and recessive. Cells harboring either mutation stop growing only after 2-3 generations at 37 degrees C. Import of the F1 beta subunit at 37 degrees C is more than 250 times slower in mas1 and 15 times slower in mas2 than in wild-type cells. At 23 degrees C, import occurs with similar rates in mutant and wild-type cells. The two mutations also reduce the rate of import of other proteins; however, import of different precursors is affected to different degrees in the two strains. The temperature-sensitive step in import in both mas1 and mas2 occurs before arrival of precursors in the mitochondrial matrix.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=391582Documentos Relacionados
- Functional cooperation of mitochondrial protein import receptors in yeast.
- A mutant nuclear protein with similarity to RNA binding proteins interferes with nuclear import in yeast.
- A nuclear mutation defective in mitochondrial recombination in yeast.
- The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast.
- Identification of two erythromycin resistance mutations in the mitochondrial gene coding for the large ribosomal RNA in yeast.