Two regulatory elements for immunoglobulin kappa light chain gene expression.
AUTOR(ES)
Bergman, Y
RESUMO
By using internal deletions within a rearranged immunoglobulin kappa light chain gene, the presence of an intron regulatory sequence (enhancer) has been confirmed. Its presence is required for high-level transcription from a plasmid after transfection into myeloma cells. Transfection efficiency was monitored by the activity of a deleted H4 histone gene included in the plasmid. The intron element could be moved upstream of the gene in both orientations, fulfilling the definition of an enhancer. By using 5' deletions, a second regulatory element was located upstream of the "TATA" box, between positions -69 and -104. These two elements both are required for efficient kappa chain gene expression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392072Documentos Relacionados
- Mice completely suppressed for the expression of immunoglobulin kappa light chain.
- Transposition of two different intracisternal A particle elements into an immunoglobulin kappa-chain gene.
- Rearrangement and expression of rabbit immunoglobulin kappa light chain gene in transgenic mice.
- Accumulation of wild-type p53 protein upon gamma-irradiation induces a G2 arrest-dependent immunoglobulin kappa light chain gene expression.
- Two rearranged immunoglobulin kappa light chain genes in one mouse myeloma.
