Two specific markers for neural differentiation of embryonal carcinoma cells.
AUTOR(ES)
Eddé, B
RESUMO
Two multipotential embryonal carcinoma (EC) cell lines, 1003 and 1009, can be induced to form preferentially neural derivatives in vitro. Synthesis of specific proteins during neural differentiation was followed by two-dimensional gel electrophoresis. The comparison of protein patterns obtained with neural and non-neural derivatives of these EC cell lines indicates that two changes are specific for the neural pathway: (i) the appearance of a new beta-tubulin isoform and (ii) the accumulation of the brain isozyme of creatine phosphokinase already present in small amounts in EC stem cells. These changes were found to take place early in the course of differentiation and to occur even when neurite outgrowth was prevented.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=555309Documentos Relacionados
- Retinoic acid-induced neural differentiation of embryonal carcinoma cells.
- Integration-specific retrovirus expression in embryonal carcinoma cells.
- Multicomponent differentiation-regulated transcription factors in F9 embryonal carcinoma stem cells.
- Metabolism of retinoic acid and retinol during differentiation of F9 embryonal carcinoma cells.
- Expression of the intracisternal A-particle is elevated during differentiation of embryonal carcinoma cells.