Type II Isopentenyl Diphosphate Isomerase from Synechocystis sp. Strain PCC 6803
AUTOR(ES)
Barkley, Sam J.
FONTE
American Society for Microbiology
RESUMO
Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His6-tagged protein was produced in Escherichia coli and purified by Ni2+ chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg2+ for activity; KmIPP was 52 μM, and kcatIPP was 0.23 s−1.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=529070Documentos Relacionados
- Tocopherols Protect Synechocystis sp. Strain PCC 6803 from Lipid Peroxidation1
- Purification and properties of glutamine synthetases from the cyanobacteria Synechocystis sp. strain PCC 6803 and Calothrix sp. strain PCC 7601.
- Identification and nitrogen regulation of the cyanase gene from the cyanobacteria Synechocystis sp. strain PCC 6803 and Synechococcus sp. strain PCC 7942.
- Multiple Light Inputs Control Phototaxis in Synechocystis sp. Strain PCC6803†
- In Vivo Role of Catalase-Peroxidase in Synechocystis sp. Strain PCC 6803