Ultrastructural and physiological changes occurring upon germination and outgrowth of Azotobacter vinelandii cysts.
AUTOR(ES)
Lin, L P
RESUMO
Dormant cysts of Azotobacter vinelandii germinated at 30 degrees C in Burk nitrogen-free media containing 1% glucose. Samples taken at intervals and examined by electron microscopy revealed that as germination progressed, vesicle-like and fibrillar structures became visible in the intine region. Lamellae associated with the cell membrane appeared in the central body at 6 h post-initiation of germination. Both electron micrographic and chemical analysis showed that the poly-beta-hydroxybutyrate content of cysts decreased significantly after 4 h of germination. Dormant cysts were resistant to sonic oscillation, but this property was lost during their conversion to metabolically active vegetative cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=222425Documentos Relacionados
- Isolation and characterization of several unique lipids from Azotobacter vinelandii cysts.
- Germination of Azotobacter vinelandii Cysts: Sequence of Macromolecular Synthesis and Nitrogen Fixation1
- Unique lipids in Azotobacter vinelandii cysts: synthesis, distribution, and fate during germination.
- Ultrastructural Changes Associated with Germination and Outgrowth of an Appendage-Bearing Clostridial Spore
- Encystment and germination in Azotobacter vinelandii.
