Ultrastructural localization of characterized antigens of Brucella abortus and distribution among different biotypes.
AUTOR(ES)
Raybould, T J
RESUMO
The distribution of characterized antigens in extracts of representative strains of Brucella abortus biotypes 1 to 9 was investigated by hemagglutination and precipitation reactivity. An antigen designated X was present in high concentrations in sodium dodecyl sulfate extracts of all smooth strains examined, but in low concentrations in the rough strain 45/20 extract. Antigen beta was demonstrable in 8 out of 12 smooth strains of B. abortus, but antigen gamma was detected in only 3 of these strains. Vaccine strain 19 extract contained beta antigen but no detectable gamma antigen, whereas both these antigens were present in rough strain 45/20 at slightly lower concentrations that in strain 544/W extract. Immunoenzyme histochemical studies performed with an electron microscope showed that antigens beta, gamma, and X were all situated in the outer membrane envelope of the cell wall of B. abortus 544/W.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=350624Documentos Relacionados
- Soluble antigens of virulent and attenuated biotypes of Brucella abortus.
- Localization of brucella antigens that elicit a humoral immune response in Brucella abortus-infected cells.
- Antigens of Brucella abortus I. Chemical and Immunoelectrophoretic Characterization
- Isolation of Two Brucella abortus Biotypes from Tissues of a Naturally Infected Cow
- Different resistance patterns of reference and field strains of Brucella abortus