Ultrastructural Studies on the Yeastlike and Mycelial Phases of Sporotrichum schenckii1

AUTOR(ES)

Lane, James W.

RESUMO

Fine details of the internal and external morphology of the yeastlike and mycelial phases of the dimorphic fungal pathogen Sporotrichum schenckii as seen in ultrathin sections are described and illustrated by electronphotomicrography. Comparisons of yeastlike phase ultrastructure were made using two different methods of fixation and embedding. The internal morphology of the two forms of yeastlike S. schenckii was in many ways similar to that of similarly dimorphic fungi and yeasts studied by other authors. However, the use of the glutaraldehyde-osmium in agar fixation technique suggests the presence of an electron transparent capsular or slime layer with associated electron dense microfibrils to be present external to the cell wall of the yeastlike phase but not the mycelial phase. Mycelial phase S. schenckii was found to contain many of the internal microstructures reported for other filamentous dimorphic fungi. Conidia production in agitated liquid culture was found to be restricted since only rare sessile conidia were observed.

Documentos Relacionados

• DIMORPHISM OF SPOROTRICHUM SCHENCKII1
• Chemical and Ultrastructural Studies on the Cell Walls of the Yeastlike and Mycelial Forms of Histoplasma farciminosum
• Deoxyribonucleic Acid Base Composition of the Yeastlike and Mycelial Phases of Histoplasma capsulatum and Blastomyces dermatitidis1
• Comparative Study of the Cell Walls of the Yeastlike and Mycelial Phases of Histoplasma capsulatum
• Ultrastructural Changes During the Yeastlike to Mycelial-Phase Conversion of Blastomyces dermatitidis and Histoplasma capsulatum