Unnatural base pairs for specific transcription
AUTOR(ES)
Ohtsuki, Takashi
FONTE
The National Academy of Sciences
RESUMO
An unnatural base pair of 2-amino-6-(N,N-dimethylamino)purine (designated as x) and pyridin-2-one (designated as y) has been developed for specific transcription. The ribonucleoside triphosphates of y and a modified y, 5-methylpyridin-2-one, are selectively incorporated into RNA opposite x in the templates by T7 RNA polymerase. In addition, the sequences of the DNA templates containing x can be confirmed by a dideoxynucleotide chain-terminator method supplemented with the deoxynucleoside triphosphate of y. The bulky dimethylamino group of x in the templates effectively eliminates noncognate pairing with the natural bases. These results enable RNA biosynthesis for the specific incorporation of unnatural nucleotides at the desired positions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=33139Documentos Relacionados
- Constructing optimal backbone segments for joining fixed DNA base pairs.
- Tools for the automatic identification and classification of RNA base pairs
- A new model for DNA containing A.T and I.C base pairs.
- A discrete region centered 22 base pairs upstream of the initiation site modulates transcription of Drosophila tRNAAsn genes.
- The Influence of Neighboring Base Pairs upon Base-Pair Substitution Mutation Rates