Unusual domains of human alphoid satellite DNA with contiguous non-satellite sequences: sequence analysis of a junction region.
AUTOR(ES)
Potter, S S
RESUMO
The sequence organization of cloned segments of Human DNA carrying unusual domains of alphoid satellite was studied by restriction mapping, electron microscopy and base sequence analysis. In some cases restriction mapping revealed the absence of the typical 340 bp EcoR 1 dimer, although blot hybridizations showed the extensive presence of alphoid satellite. A variant monomeric construction was demonstrated by DNA sequencing. Furthermore, inverted repeats within these domains were detected by electron microscopy. In one case these were shown to be the result of interruptions in the satellite sequence by members of a family of repetitive, conserved elements.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=325954Documentos Relacionados
- Nucleotide sequence heterogeneity of alpha satellite repetitive DNA: a survey of alphoid sequences from different human chromosomes.
- Characterization of cloned human alphoid satellite with an unusual monomeric construction: evidence for enrichment in HeLa small polydisperse circular DNA.
- Comparative statistics for DNA and protein sequences: single sequence analysis.
- Comparative statistics for DNA and protein sequences: multiple sequence analysis.
- Characterisation of a boundary between satellite III and alphoid sequences on human chromosome 10.