Unusual plasmid DNA organization in an octopine crown gall tumor.
AUTOR(ES)
Kwok, W W
RESUMO
A cloned tobacco crown gall tumor, 1595501, incited by A. tumefaciens strain 15955 was studied. Molecular analysis of the organization of the T-DNA by means of Southern transfer and hybridization techniques indicated that the 1595501 tumor has about 10 copies of TL DNA, five of which are complete TL DNA, whereas most octopine tumors have only one to two copies of complete TL DNA. Hybridization studies and genomic cloning indicated that some segments of the T-DNA have undergone deletions. One of the clones contained two copies of T-DNA which were inverted in orientation with respect to each other. Two left ends of TL DNA from the 1595501 tumor line and the corresponding region of the octopine plasmid were sequenced. Comparison of the various cloned T-DNA sequences with Ti-plasmid sequence indicated that while there is an association with a 25 base pair direct repeat, there is no specific set of base pairs in the T-DNA at which divergence from Ti-plasmid sequences occurred.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=341008Documentos Relacionados
- Transcription of Ti plasmid-derived sequences in three octopine-type crown gall tumor lines.
- Octopine and nopaline metabolism in Agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes.
- Sizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors.
- Promotion of Crown-Gall Tumor Growth by Lysopine, Octopine, Nopaline, and Carnosine 1
- T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells
