Upregulation of matrix synthesis in chondrocyte-seeded agarose following sustained bi-axial cyclic loading
AUTOR(ES)
Pingguan-Murphy, Belinda, Nawi, Illida
FONTE
Clinics
DATA DE PUBLICAÇÃO
2012-08
RESUMO
OBJECTIVES: The promotion of extracellular matrix synthesis by chondrocytes is a requisite part of an effective cartilage tissue engineering strategy. The aim of this in vitro study was to determine the effect of bi-axial cyclic mechanical loading on cell proliferation and the synthesis of glycosaminoglycans by chondrocytes in threedimensional cultures. METHOD: A strain comprising 10% direct compression and 1% compressive shear was applied to bovine chondrocytes seeded in an agarose gel during two 12-hour conditioning periods separated by a 12-hour resting period. RESULTS: The bi-axial-loaded chondrocytes demonstrated a significant increase in glycosaminoglycan synthesis compared with samples exposed to uni-axial or no loading over the same period (p<0.05). The use of a free-swelling recovery period prior to the loading regime resulted in additional glycosaminoglycan production and a significant increase in DNA content (p<0.05), indicating cell proliferation. CONCLUSIONS: These results demonstrate that the use of a bi-axial loading regime results in increased matrix production compared with uni-axial loading.
Documentos Relacionados
- Numerical stress-life curves for the AISI 4340 steel using two sets of materials properties and different bi-axial stress ratios
- Effect of Semi-Apical Angle on the Crush Mechanics of Metallic Bi-Tubular Tubes under Dynamic Axial Loading
- Instability of a square sheet under symmetric biaxial loading
- Optimization of reinforced concrete polygonal sections under biaxial bending with axial force
- EXPERIMENTAL INVESTIGATION ON CONCRETE FILLED STEEL TUBULAR COLUMN TO FOUNDATION CONNECTIONS SUBJECTED TO COMBINED AXIAL AND LATERAL CYCLIC LOADING