Upstream DNA sequences required for tissue-specific expression of the HLA-DR alpha gene.
AUTOR(ES)
Sherman, P A
RESUMO
We have used in vitro deletion mutagenesis in combination with DNA transfection to search for cis-acting regulatory elements involved in the tissue-specific expression of a human class II major histocompatibility complex gene. A 140-base-pair 5' flanking fragment that contains the class II box consensus sequences and an octamer sequence (ATTTGCAT) confers tissue specificity on the promoter of the HLA-DR alpha gene. Recombinant DNA plasmids containing this DR alpha gene segment fused to the coding sequence of the bacterial chloramphenicol acetyltransferase gene are expressed at higher levels in human B-cell lines than in human T-cell lines. We have demonstrated that the most 5' of the class II boxes is essential for tissue-specific DR alpha promoter function. In addition, using an electrophoretic mobility shift assay to identify DNA binding proteins, we have detected binding of nuclear proteins to DNA probes containing the class II boxes and the octamer sequence. A protein that binds to the octamer is present at higher levels in nuclear extracts of B-cell lines than in other cell lines examined. This protein may be important for the tissue-specific expression of the HLA-DR alpha gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=305063Documentos Relacionados
- DNA methylation and expression of HLA-DR alpha.
- B-cell-specific and interferon-gamma-inducible regulation of the HLA-DR alpha gene.
- Tissue-specific expression of the rat galanin gene.
- HLA-DR expression and soluble HLA-DR levels in septic patients after trauma.
- DNA sequences responsible for tissue-specific expression of a chicken alpha-crystallin gene in mouse lens cells.