Uridine Diphosphate-4-Keto-Glucose, an Intermediate in the Uridine Diphosphate-Galactose-4-Epimerase Reaction
AUTOR(ES)
Maitra, Utpalendu S.
RESUMO
When UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli is incubated with UDP-galactose, then reduced with NaB3H4, label is found in UDP-glucose and UDP-galactose. Enzymatic and chemical degradation demonstrates that the label is bound to carbon 4 of the glycosyl moieties. These results provide direct evidence for the existence of UDP-4-keto-glucose as an enzyme-bound intermediate in the epimerization reaction, and they exclude the formation of a 3-keto intermediate.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389495Documentos Relacionados
- Nucleoside Diphosphate-sugar 4-Epimerases I. Uridine Diphosphate Glucose 4-Epimerase of Wheat Germ 1
- Subunit association and catalytic activity of uridine diphosphate galactose-4-epimerase from yeast.
- Nucleoside Diphosphate-Sugar 4-Epimerases: II. Uridine Diphosphate Arabinose 4-Epimerase of Wheat Germ 1
- Bacteriophage Receptor Development and Synthesis of O-Specific Side Chains After Addition of d-Galactose to the Uridine Diphosphate-Galactose-4-Epimeraseless Mutant Salmonella typhimurium LT2-M1
- Activity of Uridine Diphosphate N-Acetylglucosamine-4-Epimerase During Spherulation of Physarum polycephalum