Use of agarose gel electrophoresis of plasmid deoxyribonucleic acid to fingerprint gram-negative bacilli.

AUTOR(ES)

Schaberg, D R

RESUMO

Agarose gel electrophoresis of the plasmid deoxyribonucleic acids from 60 gram-negative bacilli recovered during investigations of nosocomial epidemics was used to fingerprint the strains. This method was as specific at differentiating bacterial strains as more conventional phenotyping methods. In all cases, plasmid band fingerprints of epidermic strains isolates were identical whereas coisolate plasmid deoxyribonucleic acid patterns were different. Agarose gel electrophoresis of plasmid deoxyribonucleic acid is proposed as a method which can be used in conventional microbiology laboratories as an adjunct to or, possibly, replacement for other methods of identifying bacterial strains.

Documentos Relacionados

• autoSCAN-4 system for identification of gram-negative bacilli.
• Moxalactam (LY127935) in treatment of meningitis due to gram-negative bacilli.
• Effects of antimicrobial agents fed to chickens on some gram-negative enteric bacilli.
• Production of common enterobacterial antigen by pigment-producing, gram-negative bacilli.
• Evaluation of PASCO MIC-ID system for identifying gram-negative bacilli.