Use of anti-idiotypic antibodies to identify a receptor for the T-cell I-J determinant.

AUTOR(ES)

Zupko, K

RESUMO

In order to identify the molecule(s) interacting with the I-J determinant on suppressor T cells, we have generated two anti-idiotypic sera: one to monoclonal anti-I-Jd antibody and one to monoclonal anti-I-Jk antibody. These antisera specifically block suppressor T-cell function in a genetically restricted manner and have no effect on helper T-cell activation. Both recognize a marker on primary monocytes and B cells but not on T cells. A myeloma cell line bearing this marker has been identified. Therefore, these antisera may recognize a molecule on cells interacting with suppressor T cells that is involved in mediating suppressor T-cell activity. The relationship between the T-cell I-J determinant and the molecule identified by the anti-idiotype may be similar to the relationship between the receptor on helper T cells and Ia molecules.

Documentos Relacionados

• Use of anti-idiotypic antibodies as cell-surface receptor probes
• Sendai virus-specific T-cell clones: induction of cytolytic T cells by an anti-idiotypic antibody directed against a helper T-cell clone.
• Functional association of idiotypic and I-J determinants on the antigen receptor of suppressor T cells.
• Anti-idiotypic route to anti-acetylcholine receptor antibodies and experimental myasthenia gravis.
• Anti-idiotypic route to anti-acetylcholine receptor antibodies and experimental myasthenia gravis