Use of Green Fluorescent Protein To Detect Expression of an Endopolygalacturonase Gene of Colletotrichum lindemuthianum during Bean Infection
AUTOR(ES)
Dumas, Bernard
FONTE
American Society for Microbiology
RESUMO
The 5′ noncoding region of clpg2, an endopolygalacturonase gene of the bean pathogen Colletotrichum lindemuthianum, was fused to the coding sequence of a gene encoding a green fluorescent protein (GFP), and the construct was introduced into the fungal genome. Detection of GFP accumulation by fluorescence microscopy examination revealed that clpg2 was expressed at the early stages of germination of the conidia and during appressorium formation both in vitro and on the host plant.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=91249Documentos Relacionados
- Use of Green Fluorescent Protein To Assess Urease Gene Expression by Uropathogenic Proteus mirabilis during Experimental Ascending Urinary Tract Infection
- Bean reactions to 24 pathotypes of Colletotrichum lindemuthianum.
- Use of Green Fluorescent Protein To Tag and Investigate Gene Expression in Marine Bacteria
- A Cell Wall-degrading Endopolygalacturonase Secreted by Colletotrichum lindemuthianum1
- Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.