Use of Molecular Beacons To Detect an Antifolate Resistance-Associated Mutation in Plasmodium falciparum

AUTOR(ES)

Durand, Rémy

FONTE

American Society for Microbiology

RESUMO

A PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum. One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.

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