Use of the Avidin-Biotin Complex for Specific Staining of Biological Membranes in Electron Microscopy*
AUTOR(ES)
Heitzmann, H.
RESUMO
To expand the electron microscopist's options in localization and visualization, a new and general staining technique has been tested. The avidin-biotin complex serves as a coupling between the electron-dense marker, ferritin, and points of interest in biological samples. When specific cellular components are tagged with biotin, those components may be visualized with ferritin-linked avidin. Because of the remarkably strong affinity of avidin and biotin (characterized by an association constant of 1015 M-1), the staining is rapid and stable.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433809Documentos Relacionados
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