Uso de etanol como modificador organico de fase movel para cromatografia liquida de alta eficiencia

AUTOR(ES)
DATA DE PUBLICAÇÃO

1999

RESUMO

The most used organic modifiers for High Performance Liquid Chromatography (HPLC) are methanol and acetonitrile. Due to good chromatographic properties, low viscosity (when mixed with water), high purity and ready availability, these solvents are preferred in chromatographic analysis. Other solvents, such ethanol, isopropanol and acetone, have been used but problems, due to difficulties in purification (resulting in high absorbance in the ultraviolet-visible detection range) and the need for higher pressures to produce reasonable flow rates (due to high viscosity when mixed with water), were observed. Today, newer equipment supports higher pressures (4x10 Pa, 6000 psi) and high purity solvents are readily available. Therefore, some less common mobile phases deserve reinvestigation. Among organic solvents available with high purity and low absorbance in UV-VIS, ethanol is an interesting solvent, especially considering that it is much less toxic than methanol and acetonitrile. In this work, mixtures of ethanol:water have been tested for HPLC, using commercial C8 and C18 chromatographic phases. The results show that ethanol is a promising mobile phase modifier for routine laboratory use, especially at higher temperatures, where the mobile phase viscosity is lower.

ASSUNTO(S)

alcool cromatografia liquida de alta eficiencia

ACESSO AO ARTIGO

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