Utility of Green Fluorescent Nucleic Acid Dyes and Aluminum Oxide Membrane Filters for Rapid Epifluorescence Enumeration of Soil and Sediment Bacteria

AUTOR(ES)

Weinbauer, Markus G.

FONTE

American Society for Microbiology

RESUMO

High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4′,6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circumvented by using green fluorescent high-affinity nucleic acid dyes and aluminum oxide membrane filters. Due to the bright staining of cells, we recommend SYBR Green II as dye.

Documentos Relacionados

• Comparison of Blue Nucleic Acid Dyes for Flow Cytometric Enumeration of Bacteria in Aquatic Systems
• Evaluation of millipore HA and HC membrane filters for the enumeration of indicator bacteria.
• Membrane Filter-Fluorescent-Antibody Method for Detection and Enumeration of Bacteria in Water
• Enumeration of High Numbers of Bacteria Using Hydrophobic Grid-Membrane Filters
• Microcolony epifluorescence microscopy for selective enumeration of injured bacteria in frozen and heat-treated foods.