Uukuniemi virus contains an RNA polymerase.

AUTOR(ES)

Ranki, M

RESUMO

An RNA-dependent RNA polymerase activity has been found associated with Uukuniemi virions. The enzyme activity is expressed only after disrupting the virions with the nonionic detergent Triton X-100 and is absolutely dependent on Mn2+, whereas Mg2+ is not required, a finding that distinguishes this polymerase from those of other enveloped minus-strand RNA viruses. Within the range pH 7.2 to 8.5 no distinct optimum was found. The optimum temperature was between 37 and 40 C. The reaction was not inhibited by actinomycin D, rifampin, or DNase, whereas RNase was completely inhibitory. The partially RNase-resistant product consisted of rather small-sized RNA, which contained sequences complementary to Uukuniemi virus RNA as shown by hybridization to the template L, M, and S RNA species of Uukuniemi virus.

Documentos Relacionados

• Promoter analysis of influenza virus RNA polymerase.
• Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase.
• Primer recognition by avian myeloblastosis virus RNA-directed DNA polymerase.
• Synthesis of novel products in vitro by an RNA-dependent RNA polymerase.
• Characterization of an Epstein-Barr virus-induced DNA polymerase.