UV-induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense
AUTOR(ES)
Kreuzaler, Fritz
RESUMO
DNAs complementary to poly(A)+ mRNAs from UV-irradiated cell suspension cultures of parsley (Petroselinum hortense) were inserted into pBR322 and used to transform Escherichia coli strain RR1. A clone containing a DNA complementary to chalcone synthase mRNA was identified by hybrid-selected and hybrid-arrested translation. Large and rapid changes in the amount of chalcone synthase mRNA in response to irradiation of the cells was detected by RNA blot hybridization experiments. The pattern of changes coincided with that previously determined for the rate of chalcone synthase synthesis as measured either in vivo or with polyribosomal mRNA in vitro. The results are consistent with the hypothesis that induction of chalcone synthase by UV light is due to a transient increase in the rate of synthesis of chalcone synthase mRNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393872Documentos Relacionados
- A model for UV-induction of skin cancer
- UV-induction of keratinocyte endothelin-1 downregulates E-cadherin in melanocytes and melanoma cells
- Dispensable role of the NF-kappa B sites in the UV-induction of the HIV-1 LTR in transgenic mice.
- Differential induction of chalcone synthase mRNA activity at the onset of phytoalexin accumulation in compatible and incompatible plant-pathogen interactions
- In situ localization of light-induced chalcone synthase mRNA, chalcone synthase, and flavonoid end products in epidermal cells of parsley leaves
