Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella
AUTOR(ES)
Bogdanovich, T.
FONTE
American Society for Microbiology
RESUMO
A real-time, genus-specific 5′ nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did not detect non-Brucella strains (n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=404622Documentos Relacionados
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