Valinomycin-Induced Uptake of Potassium in Membrane Vesicles from Escherichia coli

AUTOR(ES)

Bhattacharyya, Pinakilal

RESUMO

Osmotically shocked Escherichia coli and membrane vesicle ghosts from E. coli cells have lost the ability to accumulate potassium by active transport. The addition of valinomycin to the membrane ghosts restores the capacity to accumulate radioactive 42K and 86Rb by a temperature- and energy-dependent process. Membrane vesicles prepared from mutants of E. coli altered in potassium transport show defects in the valinomycin-stimulated accumulation of 42K that are related to the defects in the intact cells.

Documentos Relacionados

• Valinomycin-induced cation transport in vesicles does not reflect the activity of K+ transport systems in Escherichia coli.
• Tobramycin uptake in Escherichia coli membrane vesicles.
• Active uptake of tetracycline by membrane vesicles from susceptible Escherichia coli.
• Molecular structure of membrane vesicles from Escherichia coli
• Sodium-stimulated glutamate uptake in membrane vesicles of Escherichia coli: the role of ion gradients.