Vernonia scorpioides ethyl acetate III subfraction effect evaluation in model of in vivo and in vitro murine melanoma / Avaliação dos efeitos da subfração Acetato de etila III de Vemonia scorpioides em modelo de melanoma murino in vivo e in vitro
AUTOR(ES)
Luise Zozula Blind Carrenho
DATA DE PUBLICAÇÃO
2009
RESUMO
Vernonia scorpioides has been tradionally used in Brazil in order to treat skin disorders and chronic wounds, such as leg ulcers in result to diabetes. The present study investigated the ethyl acetate III subfraction effect obtained from fresh leaves of Vernonia scorpioides in vitro, where B16F10 murine melanoma cells were submitted to treatment with several concentrations during 24, 48, 72 and 96 hours, to analyze citotoxicity subfraction before the cells. A high citotoxicity level of subfraction could be noticed killing cells above 5 μg/ml concentration, the cells which received 1 g/ml treatment have not suffered total cellular death. This death was elucidated with acridine orange/ethidium bromide staining, a deeper study of the cells was performed with electron microscopy, the action of ethyl acetate III subfraction in the 5mg/Kg concentration in C57BL6 mice was investigated in the in vivo study so that it is possible to verify the action of the subfraction seven days of treatment after ten days of inoculation, single dose of prophylactic treatment on the first day of inoculation and precocious treatment after 48 hours of inoculation with 48-hour intervals. The animals received subcutaneously 1,5 x 105 B16F10 murine melanoma cells. Tumor death is noticeable in the area where the ethyl acetate III subfraction could disseminate inside, a comprehensive necrosed area was observed, the sooner and more precocious the treatment the better the tumor will react to the ethyl acetate III subfraction, the only organ that reacts is the spleen which presents itself very reactive to the size of the tumor, the other organs do not display wounds, the subfraction did not cause apparent nefrotoxicity and hepatoxicity.
ASSUNTO(S)
vernonia scorpioides ethyl acetate iii subfraction b16f10 subfração acetato de etila iii vernonia scorpioides b16f10
Documentos Relacionados
- Ação citotóxica da sub-fração 2125 de Vernonia scorpioides contra células do Sarcoma 180 em camundongos
- Avaliação in vitro do mecanismo de ação citotóxica de lactonas sesquiterpênicas e outras substâncias isoladas de Vernonia scorpioides (LAM) PERS
- Effects of the topical application of an ethyl acetate fraction from Vernonia scorpioides on excisional wounds infected with Staphylococcus aureus in rats
- Efeito do extrato acetato de etila de Cocos nucifera Linn (Palmae) sobre a resposta inflamatória e sobre a resposta imunológica in vivo.
- Avaliação das atividades linfoproliferativa e citotóxica dos extratos metanólicos e frações de Ipomoea pes-caprae e Vernonia scorpioides
