Viral determinants of rotavirus pathogenicity in pigs: production of reassortants by asynchronous coinfection.

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A porcine rotavirus (prv), variant 4F, isolated in tissue culture from the feces of a Chinese pig with diarrhea, was found to have become highly pathogenic when passaged in gnotobiotic piglets (J. C. Bridger, B. Burke, G. M. Beards, and U. Desselberger, J. Gen. Virol. 73:3011-3015, 1992). Comparison with the closely related pig-apathogenic variant prv 4S suggested the outer capsid protein VP4 (encoded by RNA 4) of prv 4F as a determinant for pathogenicity (B. Burke, J. C. Bridger, and U. Desselberger, J. Gen. Virol. 75:2205-2212, 1994; B. Burke, J. C. Bridger, and U. Desselberger, Virology 202:754-759, 1994). In order to provide more direct evidence, the pathogenic prv 4F variant which grows and forms plaques poorly in tissue culture was reassorted with the well-tissue-culture-adapted, pig-apathogenic bovine rotavirus (brv; UK Compton strain). After asynchronous coinfection of cell cultures (first prv 4F, followed by brv 6 to 12 h later), several reassortants were isolated containing RNA 4 of prv 4F either alone (isolate B-F4) or together with one or two other genes of 4F in the genetic background of brv. Backcrossing of the monoreassortant B-F4 with prv 4S yielded a monoreassortant, S-F4, which carries RNA 4 of the 4F variant in the genetic background of prv 4S. The in vitro growth characteristics of these reassortants were analyzed, and the roles of VP4 in plaque formation and growth kinetics in cell culture were confirmed. The monoreassortant S-F4 and the parental viruses prv 4F and prv 4S are currently being tested for pathogenicity in gnotobiotic piglets (J. C. Bridger, G. Tauscher, and U. Desselberger, unpublished data).

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