Viral protein synthesis in Friend erythroleukemia cell lines.

AUTOR(ES)

Racevskis, J

RESUMO

Viral protein synthesis was studied in two Friend virus-induced erythroleukemia cell lines (Ostertag cell lines FSD1-F4 and B8) by the technique of immuno-precipitation with monospecific antisera to the major envelope glycoprotein gp70 and major core protein p30. One of the cell lines (F4) releases active Friend virus complex to the growth medium, where release of virus from the other cell line (B8) is barely or nondetectable. It was found that in the nonproducer cell line B8, a large-molecular-weight protein of about 65,000 containing p30 antigenic determinants is synthesized, yet no p30 is produced upon prolonged incubation and chase, suggesting that this might be the actual lesion that prevents mature virus production by these cells. In both cell lines, the predominant protein species that is immunoprecipitated with monospecific anti-gp70 serum is a protein of 55,000 to 60,000 daltons that is labeled with glucosamine to a much lesser extent that gp70 and appears to become heterogeneous with time. Large amounts of gp70 can be detected in the cell-free medium, but none of the unstable species of 55,00 to 60,000 molecular weight.

Documentos Relacionados

• Viral protein expression in producer and nonproducer clones of friend erythroleukemia cell lines.
• Virus production and hemoglobin synthesis in variant lines of dimethyl sulfoxide-treated Friend erythroleukemia cells.
• Viral DNA in polyoma- and SV40-transformed cell lines.
• Synthesis of a testosterone-dependent secretory protein by rat seminal vesicle-derived cell lines.
• Evidence for H-2-linked control of retrovirus production in Friend virus-induced tumor cell lines.