Viral Replicase Gene Products Suffice for Coronavirus Discontinuous Transcription
AUTOR(ES)
Thiel, Volker
FONTE
American Society for Microbiology
RESUMO
We have used vaccinia virus as a vector to clone a 22.5-kbp cDNA that represents the 5′ and 3′ ends of the human coronavirus 229E (HCoV 229E) genome, the HCoV 229E replicase gene, and a single reporter gene (coding for green fluorescent protein [GFP]) located downstream of a regulatory element for coronavirus mRNA transcription. When RNA transcribed from this cDNA was transfected into BHK-21 cells, a small percentage of cells displayed strong fluorescence. A region of the mRNA encoding GFP was amplified by PCR and shown to have the unique mRNA leader-body junction indicative of coronavirus-mediated transcription. These data show that the coronavirus replicase gene products suffice for discontinuous subgenomic mRNA transcription.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=114390Documentos Relacionados
- Two murine coronavirus genes suffice for viral RNA synthesis.
- Evidence for coronavirus discontinuous transcription.
- Interactions between coronavirus nucleocapsid protein and viral RNAs: implications for viral transcription.
- Discontinuous transcription generates heterogeneity at the leader fusion sites of coronavirus mRNAs.
- Identification of Severe Acute Respiratory Syndrome Coronavirus Replicase Products and Characterization of Papain-Like Protease Activity