Visualization of Protein-RNA Interactions in Cytoplasmic Polyhedrosis Virus
AUTOR(ES)
Zhang, H.
FONTE
American Society for Microbiology
RESUMO
Unlike the multiple-shelled organization of other Reoviridae members, cytoplasmic polyhedrosis virus (CPV) has a single-shelled capsid. The three-dimensional structures of full and empty CPV by electron cryomicroscopy show identical outer shells but differ inside. The outer surface reveals a T=1 icosahedral shell decorated with spikes at its icosahedral vertices. The internal space of the empty CPV is unoccupied except for 12 mushroom-shaped densities attributed to the transcriptional enzyme complexes. The ordered double-stranded RNA inside the full capsid forms spherical shells spaced 25 Å apart. The RNA-protein interactions suggest a mechanism for RNA transcription and release.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=103988Documentos Relacionados
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