Vitamin D metabolites change the phenotype of monoblastic U937 cells.
AUTOR(ES)
Dodd, R C
RESUMO
U937 is a human-derived lymphoma cell line that has monoblastic properties and high-affinity receptors for 1 alpha,-dihydroxyvitamin D3. Incubation of these cells with the vitamin D metabolite at 10 nM for 5 days produced marked stimulation in adherence and ingestion of Staphylococcus aureus (645% of control) and of C3b receptor (CR1) expression (292% of control) and a slight increase in hexose monophosphate shunt activity without changing cell growth rates or Fc fragment receptor expression. The changes in cellular association of S. aureus and the CR1 were detected as early as 48 hr of incubation and peaked between 3 and 5 days. Similar changes in the CR1 were induced by 25-hydroxy- and 24,25-dihydroxyvitamin D3 at micromolar concentrations. Dexamethasone, hydrocortisone, and progesterone had no effect on CR1 expression. U937 cells incubated in the presence of vitamin D metabolites exhibited a change in their phenotype. These results suggest that vitamin D metabolites may contribute to monocyte/macrophage differentiation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389987Documentos Relacionados
- Characterization of the adhesion of the human monocytic cell line U937 to cultured endothelial cells.
- Interferon gamma drastically modifies the regulation of interleukin 1 genes by endotoxin in U937 cells.
- Role of endogenous ceruloplasmin in low density lipoprotein oxidation by human U937 monocytic cells.
- Upstream regions of the c-jun promoter regulate phorbol ester-induced transcription in U937 leukemic cells.
- Autogenous production of interferon-beta switches on HLA genes during differentiation of histiocytic lymphoma U937 cells.