Whole cells in enantioselective reduction of benzyl acetoacetate
AUTOR(ES)
Ribeiro, Joyce Benzaquem, Ramos, Aline de Souza, Lopes, Raquel de Oliveira, Silva, Gabriela Veloso Vieira da, Souza, Rodrigo Octavio Mendonça Alves de
FONTE
Braz. J. Microbiol.
DATA DE PUBLICAÇÃO
2014-09
RESUMO
The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.
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