Whole genome analysis: Experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR
AUTOR(ES)
Lashkari, Deval A.
FONTE
The National Academy of Sciences of the USA
RESUMO
The recent ability to sequence whole genomes allows ready access to all genetic material. The approaches outlined here allow automated analysis of sequence for the synthesis of optimal primers in an automated multiplex oligonucleotide synthesizer (AMOS). The efficiency is such that all ORFs for an organism can be amplified by PCR. The resulting amplicons can be used directly in the construction of DNA arrays or can be cloned for a large variety of functional analyses. These tools allow a replacement of single-gene analysis with a highly efficient whole-genome analysis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=22974Documentos Relacionados
- Microarray Analysis: Genome-scale hypothesis scanning
- Representational Oligonucleotide Microarray Analysis: A High-Resolution Method to Detect Genome Copy Number Variation
- Parallel analysis of genetic selections using whole genome oligonucleotide arrays
- Genome Analysis: Genes and Phenotypes
- Prediction of Gene Function by Genome-Scale Expression Analysis: Prostate Cancer-Associated Genes