Yeast chromatin is uniformly digested by DNase-I.
AUTOR(ES)
Lohr, D
RESUMO
The DNase I (EC 3.1.21.1) sensitivity of transcribed yeast chromatin has been examined. We find that, in contrast to chromatin from higher eukaryotes, transcribed yeast chromatin and total yeast chromatin are equally sensitive to DNase I digestion. We interpret these results to mean that the entire yeast genome exists in a state that represents a restricted proportion of total chromatin in higher eukaryotes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411558Documentos Relacionados
- Genome-wide identification of DNaseI hypersensitive sites using active chromatin sequence libraries
- DNA associated with hyperacetylated histone is preferentially digested by DNase I.
- DNaseI sensitivity of the rat albumin and alpha-fetoprotein genes.
- PCR-aided DNaseI footprinting of single copy gene sequences in permeabilized cells.
- Random recombination of antibody single chain Fv sequences after fragmentation with DNaseI in the presence of Mn2+.