Yeast mitochondrial genomes consisting of only A.T base pairs replicate and exhibit suppressiveness.
AUTOR(ES)
Fangman, W L
RESUMO
Mutants, called p-, that result from extensive deletions of the 75-kilobase Saccharomyces cerevisiae mitochondrial genome arise at high frequency. The remaining mitochondrial DNA is amplified in the p- cells, often as head-to-tail multimers, producing a cell with the normal amount of mitochondrial DNA. In matings, some of these p- mutants exhibit zygotic hypersuppressiveness, excluding the wild-type mitochondrial genome (p+) from all the diploids that are produced. From a hypersuppressive p- strain, we isolated two mutants with reduced suppressiveness. These mutants, one moderately suppressive and one nonsuppressive, retain only 89 and 70 base pairs, respectively, of the wild-type mitochondrial genome. Their sequences consist of 100% A . T base pairs. Replication of DNA in the mitochondrion, formation and amplification of new deletion genomes, and exhibition of suppressiveness do not require a single G . C base pair.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392096Documentos Relacionados
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