Yeast pre-mRNA splicing requires a minimum distance between the 5' splice site and the internal branch acceptor site.
AUTOR(ES)
Thompson-Jäger, S
RESUMO
We have generated several deletions within the intron of a yeast actin gene construct which have lead to different splicing efficiencies as measured by Northern blot (RNA blot) and primer extension analyses. Our data especially demonstrate that a minimum distance from the 5' splice site to the internal branch acceptor site is required for accurate and efficient splicing. In a construct in which splicing was completely abolished, splicing could be restored by expanding the distance from the 5' splice site to the internal branch acceptor site with heterologous sequences. Alternative splicing, i.e., exon skipping and the use of a cryptic 5' splice site, was observed when the mRNA precursor was derived from a tandem repeat of a truncated intron with flanking exon sequences.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=368070Documentos Relacionados
- Splicing and spliceosome formation of the yeast MATa1 transcript require a minimum distance from the 5' splice site to the internal branch acceptor site.
- Spatial Organization of Protein-RNA Interactions in the Branch Site-3′ Splice Site Region during pre-mRNA Splicing in Yeast
- Structure of a pre-mRNA branch point/3' splice site region.
- The human splicing factor ASF/SF2 can specifically recognize pre-mRNA 5' splice sites.
- Mechanism for cryptic splice site activation during pre-mRNA splicing.