Z-DNA binding protein from chicken blood nuclei.
AUTOR(ES)
Herbert, A G
RESUMO
A protein (Z alpha) that appears to be highly specific for the left-handed Z-DNA conformer has been identified in chicken blood nuclear extracts. Z alpha activity is measured in a band-shift assay by using a radioactive probe consisting of a (dC-dG)35 oligomer that has 50% of the deoxycytosines replaced with 5-bromodeoxycytosine. In the presence of 10 mM Mg2+, the probe converts to the Z-DNA conformation and is bound by Z alpha. The binding of Z alpha to the radioactive probe is specifically blocked by competition with linear poly(dC-dG) stabilized in the Z-DNA form by chemical bromination but not by B-form poly(dC-dG) or boiled salmon-sperm DNA. In addition, the binding activity of Z alpha is competitively blocked by supercoiled plasmids containing a Z-DNA insert but not by either the linearized plasmid or by an equivalent amount of the parental supercoiled plasmid without the Z-DNA-forming insert. Z alpha can be crosslinked to the 32P-labeled brominated probe with UV light, allowing us to estimate that the minimal molecular mass of Z alpha is 39 kDa.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=46295Documentos Relacionados
- Transcription is associated with Z-DNA formation in metabolically active permeabilized mammalian cell nuclei.
- Zuotin, a putative Z-DNA binding protein in Saccharomyces cerevisiae.
- Are many Z-DNA binding proteins actually phospholipid-binding proteins?
- A role for Z-DNA binding in vaccinia virus pathogenesis
- Z-DNA in transcriptionally active chromosomes.