Zeste-mediated activation by an enhancer is independent of cooperative DNA binding in vivo

AUTOR(ES)

Laney, Jeffrey D.

FONTE

The National Academy of Sciences of the USA

RESUMO

It is not clear how transcription factors bound at distal enhancer and proximal promoter sequences cooperate to stimulate transcription in vivo. To distinguish between different models for the action of enhancer elements, we have directly measured DNA binding of the Drosophila activator zeste by in vivo UV crosslinking. Experiments in Drosophila embryos show that binding of zeste protein to either the proximal promoter of the Ultrabithorax (Ubx) gene or to a Ubx enhancer element does not require the presence of the other element. However, significant transcription is observed only when both elements are present and bound by zeste. The results indicate that stimulation by an enhancer can occur by a mechanism other than increasing the occupancy of an activator to binding sites near the start site of transcription.

Documentos Relacionados

• Chromatin inheritance upon Zeste-mediated Brahma recruitment at a minimal cellular memory module
• Modulation of promoter occupancy by cooperative DNA binding and activation-domain function is a major determinant of transcriptional regulation by activators in vivo.
• Distance-Independent Inactivation of an Enhancer by the Suppressor of Hairy-Wing DNA-Binding Protein of Drosophila
• Redox activation of Fos-Jun DNA binding activity is mediated by a DNA repair enzyme.
• DNA bending is induced in an enhancer by the DNA-binding domain of the bovine papillomavirus E2 protein.