B Galactosides
Mostrando 1-12 de 17 artigos, teses e dissertações.
-
1. Caracterização bioquímica e molecular da ß-Galactosidade durante a maturação de frutos de coffea arabica
ß-galactosidases are a class of glycosyl-hydrolases that act on the plant cell primary walls, hydrolyzing ß-D-galactose at the nonreducing ends of ß-D-galactosides present in several biological molecules. Initially a characterization of the monosaccharides present in the primary wall of the pericarp and endosperm of Coffea arabica fruits at different ripe
Publicado em: 2011
-
2. Engagement of galectin-1 in mild hyperhomocysteinemia-induced vascular injury in mice. / Participação da galectina-1 na lesão vascular induzida e camundongos com hiperhomocisteinemia moderada
Galectin-1 (Gal-1) belongs to the lectin family of -galactosides-binding proteins and can be expressed by several cell types, including endothelial cells and vascular smooth cells. This endogenous lectin has immunological and anti-inflammatory properties dependent of essential cellular processes, including activation, differentiation, survival, phagocytosis
Publicado em: 2007
-
3. Purificação, caracterização, clonagem e seqüenciamento de β-glicosidades de Tenebrio molitor (Coleoptera) / Purification, characterization, cloning and sequencing of β-glycosidases from Tenebrio molitor (Coleoptera)
In the midgut lumen of Tenebrio molitor larvae there are 4 β-glycosidases (named 1, 2, 3a and 3b), not present in the animal food. They were purified with electrophoresis, ion exchange and hydrophobic chromatographies. β-glycosidase 1 (relative molecular weight - Mr 59,000) is unstable at 30°C but is stabilized by substrates. The enzyme hydrolyses
Publicado em: 2001
-
4. Gargoylism: hydrolysis of β-galactosides and tissue accumulation of galactose- and mannose-containing compounds
The sugars present in hydrolyzed extracts of human liver and brain were analyzed by gasliquid chromatography after conversion to their alditol acetates. The samples analyzed were obtained from control subjects, patients with gargoylism, and patients with a few other kinds of storage disorders. Accumulation of galactose was demonstrated in the liver and the b
-
5. Gas-Liquid Chromatographic Analysis of Indole-3-acetic Acid Myoinositol Esters in Maize Kernels 1
An improved method of fractionating the myoinositol esters of indoleacetic acid (IAA) from maize kernels by gas-liquid chromatography has been developed. Mass spectrometry was employed as an aid in identification of the esters. Maize kernels contain three groups of esters of IAA: (a) IAA myoinositols, (b) IAA myoinositol arabinosides, and (c) IAA myoinositol
-
6. Phage display of ricin B chain and its single binding domains: system for screening galactose-binding mutants.
We demonstrate that the B chain of ricin toxin preserves its lectin activity when expressed as a fusion protein on the surface of fd phage. Moreover, B chain, which folds into two topologically similar globular domains, can be dissected into amino-terminal and carboxyl-terminal domains to form single binding domains (SBDs) of B chain, each of which displays
-
7. Regulation of lactose-phosphoenolpyruvate-dependent phosphotransferase system and beta-D-phosphogalactoside galactohydrolase activities in Lactobacillus casei.
The lactose-phosphoenolpyruvate-dependent phosphotransferase system (lac-PTS) and beta-D-phosphogalactoside galactohydrolase (P-beta-gal) mediate the metabolism of lactose by Lactobacillus casei. Starved cells of L. casei contained a high intracellular concentration of phosphoenolpyruvate, and this endogenous energy reserve facilitated characterization of ph
-
8. α-Galactoside Uptake in Rhizobium meliloti: Isolation and Characterization of agpA, a Gene Encoding a Periplasmic Binding Protein Required for Melibiose and Raffinose Utilization
Rhizobium meliloti can occupy at least two distinct ecological niches; it is found in the soil as a free-living saprophyte, and it also lives as a nitrogen-fixing intracellular symbiont in root nodules of alfalfa and related legumes. One approach to understanding how R. meliloti alters its physiology in order to become an integral part of a developing nodule
American Society for Microbiology.
-
9. Properties of beta-galactosidase III: implications for entry of galactosides into Klebsiella.
Klebsiella sp. strain CT-200 lacks both its plasmid-borne lac operon, which specifies beta-galactosidase I, and its chromosomal lac operon, which specifies beta-galactosidase II, but it expresses a gene for a third beta-galactosidase, beta-galactosidase III, constitutively. CT-200 was examined to determine whether there was a beta-galactoside permease associ
-
10. Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli.
The conjugative plasmid pRSD2 carries a raf operon that encodes a peripheral raffinose metabolic pathway in enterobacteria. In addition to the previously known raf genes, we identified another gene, rafY, which in Escherichia coli codes for an outer membrane protein (molecular mass, 53 kDa) similar in function to the known glycoporins LamB (maltoporin) and S
-
11. Acylated Cholesteryl Galactosides Are Specific Antigens of Borrelia Causing Lyme Disease and Frequently Induce Antibodies in Late Stages of Disease
Borrelia burgdorferi sensu lato is the causative agent of Lyme disease (LD), an infectious disease occurring in North America, Europe, and Asia in different clinical stages. B. burgdorferi sensu lato encompasses at least 12 species, with B. burgdorferi sensu stricto, B. garinii, and B. afzelii being of highest clinical importance. Immunologic testing fo
American Society for Biochemistry and Molecular Biology.
-
12. Purification and characterization of a Bacteroides loeschei adhesin that interacts with procaryotic and eucaryotic cells.
The adhesin of Bacteroides loeschei PK1295 that mediates coaggregation with Streptococcus sanguis 34 and hemagglutination of erythrocytes was purified to electrophoretic homogeneity. The lectinlike protein has an estimated native Mr of 450,000 and consists of six subunits of identical molecular weight (Mr 75,000). The purified adhesin appears to be a basic p