Baculovirus System
Mostrando 1-12 de 341 artigos, teses e dissertações.
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1. Solubility as a limiting factor for expression of hepatitis A virus proteins in insect cell-baculovirus system
The use of recombinant proteins may represent an alternative model to inactivated vaccines against hepatitis A virus (HAV). The present study aimed to express the VP1 protein of HAV in baculovirus expression vector system (BEVS). The VP1 was expressed intracellularly with molecular mass of 35 kDa. The VP1 was detected both in the soluble fraction and in the
Mem. Inst. Oswaldo Cruz. Publicado em: 11/07/2016
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2. Design of signal peptide bombyxin and its effect on secretory expression efficiency and levels of Helicobacter pylori urease subunit B in silkworm cells and larvae
This study employed a Bac-to-Bac/Bombyx mori bioreactor to mass-produce immunogenic urease subunit B (UreB) from Helicobacter pylori.The signal peptide bombyxin from B. mori was used to promote secretory expression to improve expression levels and was designed and integrated into the UreB gene to generate the Bacmid/BmNPV/(signal peptide)-UreB baculovirus ex
Braz. arch. biol. technol.. Publicado em: 2015-06
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3. Cloning and Functional Assessment of the Recombinant Human Hepcidin-25 in the Baculovirus Expression System
Hepcidin is the primary regulatory hormone responsible for lowering the iron content in the blood circulation. Due to its biodegradability and low cytotoxicity, hepcidin is considered as an alternative for iron chelators. The baculovirus expression system may be suitable for human hepcidin production because the expressed proteins generally exhibit proper fo
Braz. arch. biol. technol.. Publicado em: 01/08/2014
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4. Expressão de proteína antiviral de lonomia obliqua em sistema baculovírus/célula de inseto / Expression of an antiviral protein from Lonomia obliqua in baculovirus/insect cell system
A tecnologia de cultivo de celulas animais tem permitido nos ultimos anos o desenvolvimento de inumeros bioprodutos. Principalmente com interesse farmacologico, alguns desses produtos, as proteinas recombinantes, podem ser produzidas em sistemas de expressao heterologos em escala comercial. Bacterias, leveduras, celulas de mamiferos e de insetos sao alguns d
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 16/02/2012
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5. O interactoma de Stanniocalcina-1 humana sugere novas funções e vias de atuação celulares / The interactome of human Stanniocalcin-1 suggests new cellular functions and pathways
O objetivo deste projeto foi estudar genes ativados em células do estroma da medula óssea, induzidos pela co-cultura com blastos leucêmicos, na tentativa de uma melhor compreensão sobre o crostalk entre estas células no microambiente tumoral. Nós identificamos Stanniocalcina-1 (STC1) como um potencial marcador molecular do microambiente tumoral, uma ve
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 19/08/2011
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6. Montagem de um pseudo-hantavírus quimera, contendo a nucleoproteína do vírus Araraquara e as glicoproteínas do vírus Andes, em sistema baculovírus / Assembly of a chimeric hantavirus-like particle, containing the Araraquara nucleoprotein and the Andes glycoproteins, expressed in baculovirus system
Hantaviruses, members of the Bunyaviridae family, are the infectious agents responsible for Hemorrhagic Fever with Renal Syndrome and the Hantavirus Cardiopulmonary Syndrome. The viral genome is composed by three segments of single-stranded negative-sense RNA, designated as S, M and L, which encode, respectively, the nucleoprotein, the G1 and G2 glycoprotein
Publicado em: 2010
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7. Título Principal Toxinas recombinantes Cry2Aa e Cry11A de Bacillus thuringiensis expressas em células de inseto são tóxicas para larvas de Lepidoptera e Diptera
Bacillus thuringiensis (Bt) is a Gram positive, tomopathogenic bacteria that produces crystalline inclusions called !-endotoxins or Cry proteins. These proteins are highly toxic to susceptible insects, not showing activity against others organisms. Some expression systems have been used to express crystal proteins with the aim to increase their toxicity for
Publicado em: 2009
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8. Estudos das interações da septina 4 humana / Study of Human Septin 4 interactions
Septinas são proteínas ligantes a GTP encontradas desde fungos até metazoários. A primeira função identificada para septinas foi o seu papel central na organização e dinâmica do septo de divisão de leveduras. Uma das características marcantes é que septinas se organizam em heterofilamentos de 7 a 9 nm de espessura que foram purificados de diverso
Publicado em: 2009
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9. Estudos estruturais e funcionais da proteina stanniocalcina-1 humana, um novo marcador de microambiente de leucemia / Structural and functional studies of human protein stanniocalcina-1, a new microenvironmental marker of leukemia
Staniocalcins (STCs) represent a small family of glycoprotein hormones found in all vertebrates composed by STC1 and STC2, which have been initially implicated in calcium homeostasis and also recently implicated in several other processes. Stanniocalcin-1 (STC1), the first member found, was originally discovered in bony fishes and later identified in humans
Publicado em: 2009
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10. Estudo da interação do adenovírus humano, sorotipo 41 (HAdV-41), com células permissivas. / Interaction studies of human adenovirus serotype 41 (HAdV-41) with permissive cells.
Com o objetivo de estudar a interação do HAdV-41 com células permissivas, primeiramente foi observada a cinética de infecção do HAdV-41 em células HEK-293, durante 7 dias. A seguir, as culturas foram analisadas por MCVL e por MET. O HAdV-41 apresentou um ciclo replicativo lento com liberação da progênie viral por mecanismo não lítico. A seguir, c
Publicado em: 2008
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11. Development and evaluation of immunization tools based on the adenovirus fiber knob modified with the C4 domain of HIV gp120 glycoprotein. / Desenvolvimento e avaliação de ferramentas de imunização baseadas na região globular da fibra adenoviral modificada com o domínio C4 da glicoproteína gp120 do HIV.
HIV glycoprotein gp120 has conserved domains, one of them being the C4 domain. This region is involved in the recognition of the CD4 marker in target cells and antibodies that recognize this domain can block HIV infection. Previously, the C4 domain was introduced in the adenovirus fiber knob. As the adenovirus fiber stimulates de immune system, we decided to
Publicado em: 2008
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12. Expressão recombinante da canacistatina em células de inseto.
Due to the great economic importance of sugarcane crop in Brazil, the occurrence of infections by phytopathogens leading to decreased productivity and quality remains a serious problem. As the plants have natural mechanisms of defense against the attack of fungi and insects, amongst which the inhibitors of proteases are distinguished, the use of these substa
Publicado em: 2007