Benomyl Resistance
Mostrando 1-12 de 34 artigos, teses e dissertações.
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1. Survival in soil and detection of co-transformed Trichoderma harzianum by nested PCR.
The objective of this work was to evaluate the survival of two Trichoderma harzianum co-transformants, TE 10 and TE 41, carrying genes for green fluorescent protein (egfp) and for resistance to benomyl, during four weeks in a contained soil microcosm. Selective culture media were used to detect viable fungal material, whose identity was confirmed by the obse
Pesquisa Agropecuária Brasileira. Publicado em: 2011
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2. Sensibilidade a fungicidas e adaptabilidade de Lasiodiplodia theobromae patogênico ao mamão
Application of fungicide is the main measure of management to stem-end rot and there is no information on the sensitivity and on the fitness costs arising from the reduction in sensitivity of its causal agent, Lasiodiplodia theobromae. One hundred and twenty monosporic isolates collected in producing areas of the Northeast region of Brazil, were divided into
Publicado em: 2009
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3. Inoculum production, the effect of vegetable extracts and fungicides and soybean genotypes reaction to Sclerotinia sclerotiorum / Produção de inóculo, efeito de extratos vegetais e de fungicidas e reação de genótipos de soja à Sclerotinia sclerotiorum
A podridão branca da haste causada por S. sclerotiorum vem aumentando em campos de cultivo de soja, devido ao cultivo de espécies altamente suscetíveis na safrinha e a utilização de sementes contaminadas por S. sclerotiorum. Estudos envolvendo produção de inóculo, controle alternativo e químico, metodologia de inoculação e resistência de genótip
Publicado em: 2008
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4. ResistÃncia das leveduras Kluyveromyces marxianus e Saccharomyces cerevisiae ao fungicida Benomyl
Investigations on the toxic compound-resistant phenotypes by microrganisms are regarded as relevant within the bio-technological, clinic and farming fields. In this work, we aimed to identify the co-relation between the resistance levels of yeast cells to the fungicide Benomyl and the presence of two yeast genes already related to have any influence
Publicado em: 2007
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5. Arbuscular mycorrhizal fungi communities in citrus conventional and organic farming and their interactions with Phytophthora parasitica. / Comunidades de fungos micorrízicos arbusculares nos manejos convencional e orgânico de citros e suas interações com Phytophthora parasitica.
Farmers and technicians involved with organic citriculture try to develop systems with high microbial activity in soil. In this way, they expect to obtain benefits from processes that occur in soil, as natural control of pests and diseases. However, there are few studies about the influence of this type of management on soil microbiota, specially on the arbu
Publicado em: 2004
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6. Resistance of brazilian sugarcane cultivars to ratoon stunting disease basead on the rate of stalk colonization by Leifsonia xyli subsp. Xyli. / Avaliação da resistência de variedades de cana-de-açúcar ao raquitismo-da-soqueira com base na taxa de colonização dos colmos por Leifsonia xyli subsp. xyli.
The tissue blot immunoassay (TBIA) for detecting Leifsonia xyli subsp. xyli (Lxx) colonized vascular bundles (CVB) were used in determining the reaction of 28 brazilian sugarcane cultivars to ratoon stunting disease (RSD), including CB47-355, CB41-76 e CB49-260 as resistant, intermediate and susceptible standards. For each cultivar, 16 buds were hot water tr
Publicado em: 2004
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7. Isolation of a beta-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance.
A beta-tubulin gene from a UV-irradiated benomyl-resistant mutant of Fusarium moniliforme was isolated, cloned, and sequenced. The gene encodes a 446-amino-acid polypeptide with homology to other fungal beta-tubulins. RNA blot analysis showed expression of the gene during vegetative growth and conidial germination but no expression during conidiation. A poin
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8. Genetic Transformation System for the Fungal Soybean Pathogen Cercospora kikuchii
An altered β-tubulin gene that confers resistance to the fungicide benomyl was isolated from a genomic library of a UV-induced mutant of Cercospora kikuchii and used as a selectable marker for transformation. The level of benomyl resistance conferred to the transformants was at least 150-fold greater than the intrinsic resistance of the C. kikuchii recipien
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9. Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance
Methodology was developed to isolate and regenerate protoplasts from the biocontrol fungus Gliocladium virens and to transform them to benomyl resistance with a Neurospora crassa β-tubulin gene. Southern blots demonstrated that multiple copies of the vector integrated into the chromosomal DNA of stable biotypes but not of abortive transformants. Analysis of
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10. Candida albicans gene encoding resistance to benomyl and methotrexate is a multidrug resistance gene.
Candida albicans is not inhibited by a number of drugs known to affect fungal cells. The basis for this resistance in most cases is unknown but has been attributed to the general impermeability of the fungal cell envelope. A gene (BENr) formerly shown to be responsible for the resistance of C. albicans to benomyl and methotrexate was shown in the present stu
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11. Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker.
We cloned the beta-tubulin gene of Neurospora crassa from a benomyl-resistant strain and determined its nucleotide sequence. The gene encodes a 447-residue protein which shows strong homology to other beta-tubulins. The coding region is interrupted by six introns, five of which are within the region coding for the first 54 amino acids of the protein. Intron
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12. Structure–Function Relationships in Yeast TubulinsD⃞
A comprehensive set of clustered charged-to-alanine mutations was generated that systematically alter TUB1, the major α-tubulin gene of Saccharomyces cerevisiae. A variety of phenotypes were observed, including supersensitivity and resistance to the microtubule-destabilizing drug benomyl, lethality, and cold- and temperature-sensitive lethality. Many of the
The American Society for Cell Biology.