Beta Glucosidases
Mostrando 1-12 de 29 artigos, teses e dissertações.
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1. Produção e caracterização de proteínas do complexo celulolítico de Trichoderma harzianum e T.reesei, envolvidas na hidrólise enzimática da biomassa / Production and Characterization of proteins from the cellulolytic cocktail of Trichoderma harzianum e T.reesei, involved in enzymatic hydrolysis of biomass.
Celulases têm atraído muito interesse nos últimos anos devido a sua habilidade na bioconversão de material lignocelulolítico em glucose, a qual pode, então, ser convertida a etanol por fermentação. O complexo celulolítico capaz de degradar a celulose consiste de várias enzimas (principalmente celulases e β-glucosidases) e proteínas auxiliador
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/05/2012
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2. Structural characterization of the bglH gene encoding a beta-glucosidase-like enzyme in an endophytic Bacillus pumilus strain
A beta-glucosidase-like enzyme-encoding gene (bglH) of an endophytic Bacillus pumilus strain (CL16) was cloned using a shotgun genomic library constructed in Escherichia coli. The nucleotide sequence of the entire cloned fragment (2484 bp) was determined and characterized. An incomplete open reading frame (ORF) of 534 bp (ORF1) designated bglP and a complete
Genetics and Molecular Biology. Publicado em: 2007
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3. alfa e b-glucosidases como alvos moleculares para desenvolvimento de fármacos
Glucosidases are involved in key steps in the processing of oligosaccharides by cleaving O-glucose residues. Since they catalyze breaking and transfer reactions of glucosidic groups for the normal growth and development of all the cells, defects or genetic deficiencies in these enzymes are associated with serious disorders of the carbohydrate metabolism. Thu
Química Nova. Publicado em: 2006-07
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4. Purification and characterization of digestive beta-glycosidases from Spodoptera frugiperda (Lepidoptera) / Purificação e caracterização das β-glicosidases digestivas de Spodoptera frugiperda (Lepidoptera)
Foram purificadas através de uma combinação de cromatografias as duas β- glicosidases digestivas (Mr 47.000 e 50.000 - denominadas β47 e β50, respectivamente) encontradas na larva de S. frugiperda. Experimentos de competição entre substratos e modificação química mostraram que a β47 possui dois sítios ativos. Um desses sítios de
Publicado em: 1999
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5. Distribuição intracelular de enzimas digestivas e caracterização das beta-glucosidases intestinais de Abracris flavolineata / Intracellular distribution of digestive enzymes and characterization of the digestive beta-glucosidases from Abracris flavolineata
Nas células do ceco anterior de A.flavolineata, a secreção de enzimas digestivas parece ser mediada por vesículas de secreção e é influenciada pelo tempo decorrido após a refeição. Fracionamentos subcelulares das células do ceco anterior realizados 3h após a refeição indicaram que a amilase, maltase, pNΦβglu hidrolase e aminopeptidase
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/08/1994
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6. Beta-glucoside permeases and phospho beta-glucosidases in Aerobacter aerogenes: relationship with cryptic phospho beta-glucosidases in Enterobacteriaceae.
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7. Amygdalin (Laetrile) and prunasin beta-glucosidases: distribution in germ-free rat and in human tumor tissue.
Amygdalin, the gentiobioside derivative of mandelonitrile commonly referred to as Laetrile, is presently under intensive investigation as a potential cancer chemotherapeutic agent. Because of this interest, we investigated the activity of beta-glucosidases that cleave glucose from amygdalin and from prunasin (mandelonitrile monoglucoside) in tissues from ger
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8. Cloning and sequencing of an Agrobacterium tumefaciens beta-glucosidase gene involved in modifying a vir-inducing plant signal molecule.
Induction of Agrobacterium tumefaciens virulence genes by plant phenolic compounds is essential for successful T-DNA transfer to a host plant. In Douglas fir needles, the major virulence region inducer is the glycoside coniferin (J. W. Morris and R. O. Morris, Proc. Natl. Acad. Sci. USA 87:3612-3618, 1990). Agrobacterium strains with high beta-glucosidase ac
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9. A beta-glucosidase from lodgepole pine xylem specific for the lignin precursor coniferin.
Coniferin, the glucoside of the monolignol coniferyl alcohol, accumulates to high levels in gymnosperms during spring-cambial reactivation. A cinnamyl alcohol glucoside/beta-glucosidase system is thought to play a key role in lignification by releasing the monolignol aglycones. Investigation of such an enzyme system in the xylem of Pinus contorta var latifol
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10. Genes encoding two different beta-glucosidases of Thermoanaerobacter brockii are clustered in a common operon.
A 5.9-kb fragment of chromosomal DNA coding for beta-glucosidase activity of the thermophilic anaerobe Thermoanaerobacter brockii was sequenced. Two genes, cglT and xglS, encoding a cellodextrin-cleaving beta-glucosidase and a xylodextrin-degrading xylo-beta-glucosidase, respectively, were located directly adjacent to each other. The 5' region contained two
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11. Purification, characterization, gene cloning, and sequencing of a new beta-glucosidase from Bacillus circulans subsp. alkalophilus.
An intracellular beta-glucosidase was purified from cell extracts of Bacillus circulans subsp. alkalophilus by NAD affinity and high-performance anion-exchange chromatographies. The enzyme was active against a wide range of aryl-beta-glucosides and beta-linked disaccharides. The structural gene for beta-glucosidase was cloned in Escherichia coli. The beta-gl
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12. Nucleotide sequences of Saccharomycopsis fibuligera genes for extracellular beta-glucosidases as expressed in Saccharomyces cerevisiae.
We isolated two genes for extracellular beta-glucosidase, BGL1 and BGL2, from the genomic library of the yeast Saccharomycopsis fibuligera. Gene products (BGLI and BGLII) were purified from the culture fluids of Saccharomyces cerevisiae transformed with BGL1 and BGL2, respectively. Molecular weights of BGLI and BGLII were estimated to be 220,000 and 200,000