Biolistics
Mostrando 1-12 de 13 artigos, teses e dissertações.
-
1. Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics
ABSTRACT Brachiaria brizantha is a forage grass well adapted to tropical areas and cultivated in millions of hectares in Brazil. The apomictic mode of reproduction in this species, in addition to differences in ploidy between sexual and apomictic plants, impairs crossbreeding. The development of a methodology to transform apomictic cultivars will provide an
An. Acad. Bras. Ciênc.. Publicado em: 2018-04
-
2. Introduction of the rd29A: AtDREB2A CA gene into soybean (Glycine max L. Merril) and its molecular characterization in leaves and roots during dehydration
The loss of soybean yield to Brazilian producers because of a water deficit in the 2011-2012 season was 12.9%. To reduce such losses, molecular biology techniques, including plant transformation, can be used to insert genes of interest into conventional soybean cultivars to produce lines that are more tolerant to drought. The abscisic acid (ABA)-independent
Genet. Mol. Biol.. Publicado em: 2013
-
3. Cultura de tecidos e transformação genética de espécies da família Poaceae / Tissue culture and genetic transformation of Poaceae family species
Brachiaria é um gênero de forrageiras da família Poaceae que apresenta plantas que se reproduzem por via sexual e assexualmente por apomixia,reprodução por sementes. A apomixia desperta interesse biológico e biotecnológico, pela perspectiva de levar esta característica de clonagem de plantas via sementes, a outras espécies. As cultivares plantadas d
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/07/2012
-
4. Plant tissue culture and regeneration of transgenic plants from embryogenic callus and immature leaves of sugarcane / Cultura de tecidos e regeneração de plantas transgênicas a partir de calos embriogênicos e de folhas imaturas de cana-de-açúcar
Sugarcane is a polyploidy monocot and allogamous species that has low reproductive rate due to the difficulty of flowering. Because of these genetic and physiological characteristics breeding program takes long time and demand hard labor. Alternatively, modern biotechnology approaches contribute to the development of new cultivars. In this work we studied th
Publicado em: 2010
-
5. Biological and molecular characterization of begomoviruses in soybean (Glycine max) and Euphorbia heterophylla, and RNA interference-mediated virus resistance in transgenic soybean plants / Caracterização molecular e biológica de begomovírus de soja (Glycine max) e leiteiro (Euphorbia heterophylla) e resistência a vírus mediada por RNA interferente em plantas transgênicas de soja
Molecular characterization of begomoviruses is of fundamental importance to identify the mechanisms of genetic variability of these important plant pathogens. Genome analysis allows for an understanding of mutation and recombination mechanisms which lead to evolution of new viral species. In Brazil, only a few reports of begomovirus infection of soybean have
Publicado em: 2009
-
6. Mosaico do quiabeiro : etiologia, caracterização e diversidade
Okra mosaic disease was frequently observed in the 60s in Brazil, but its importance decreased after the use of the resistant cultivar Santa Cruz 47. It was believed that this disease was caused by a begomovirus, but the etiology was never confirmed. Several okra production fields were visited in central Brazil (Federal District and Goiás state) for evaluat
Publicado em: 2008
-
7. Attacin a gene transference to plants of yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg.) by biolistics. / Transferência do gene atacina A para plantas de maracujá amarelo (Passiflora edulis Sims f. flavicarpa Deg.) por biobalística.
O Brasil é o principal produtor de maracujá amarelo. Entretanto, a produtividade é baixa, cerca de 1 0.000 t por hectare. A produção de frutos varia com o cultivar, condições climáticas, manejo e outros fatores, principalmente doenças causadas por bactérias e vírus. Metodologias de transformação genética são alternativas modernas para obter pl
Publicado em: 2002
-
8. Electroporation, an alternative to biolistics for transfection of Bombyx mori embryos and larval tissues
There are few powerful techniques available to transfect insect tissues. We previously used biolistics to transfect Bombyx mori embryos, and larval and pupal tissues (Thomas J-L et al. 2001. Journal of Insect Science 1/9, Kravariti L et al. 2001. Insect Biochemistry and Molecular Biology 31: 473–479). As the main limitation was the irregularity in results
University of Arizona Library.
-
9. Transient expression of DNA and RNA in parasitic helminths by using particle bombardment
Parasitic helminths (worms belonging to several metazoan phyla) cause considerable morbidity and mortality in humans. They are an important veterinary problem, and they result in significant economic losses in animal grazing and agriculture. Experimental studies on parasitic helminths have been limited by a lack of parasite cell lines and methods for molecul
The National Academy of Sciences.
-
10. Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA.
A transformation scheme for Cryptococcus neoformans to yield high-frequency, integrative events was developed. Adenine auxotrophs from a clinical isolate of C. neoformans serotype A were complemented by the cryptococcal phosphoribosylaminoimidazole carboxylase gene (ade2) with a biolistic DNA delivery system. Comparison of two DNA delivery systems (electropo
-
11. A new approach for the identification and cloning of genes: the pBACwich system using Cre/lox site-specific recombination
With current plant transformation methods (Agrobacterium, biolistics and protoplast fusion), insertion of DNA into the genome occurs randomly and in many instances at multiple sites. Associated position effects, copy number differences and multigene interactions can make gene expression experiments difficult to interpret and plant phenotypes less predictable
Oxford University Press.
-
12. Physical methods of nucleic acid transfer: general concepts and applications
Physical methods of gene (and/or drug) transfer need to combine two effects to deliver the therapeutic material into cells. The physical methods must induce reversible alterations in the plasma membrane to allow the direct passage of the molecules of interest into the cell cytosol. They must also bring the nucleic acids in contact with the permeabilized plas
Blackwell Publishing Ltd.