Biotinylation
Mostrando 1-12 de 103 artigos, teses e dissertações.
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1. Desenvolvimento, caracterizacao e aplicacao da matriz FTO/POLI(3-aminofenol) na deteccao de marcador de lesao cardiaca por fotoluminescencia de quantum dots
The heart is a vital organ propelling blood, that carries oxygen and nutrients throughout the body, but whenever these components do not reach it due to arterial obstruction, there is an electrical instability in the process of contraction of the muscle, causing arrhythmias that often, in less than an hour, causes acute myocardial infarction. The problem and
Publicado em: 2011
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2. Detecção condutométrica sem contato: uma nova ferramenta para monitoramento de interações biomoleculares em microssistemas analíticos / Contactless conductivity detection: a new tool for monitoring biomolecular interactions on analytical microsystems
The study reported in this thesis shows the application of a capacitively coupled contactless conductivity detection (C4D) for monitoring biomolecular interactions on analytical microsystems. Initially, the analytical performance of the microsystems fabricated in glass, poly(dimethylsiloxane) (PDMS) and polyester-toner (PT) was investigated in order to choos
Publicado em: 2008
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3. Proteomic analysis of the shistosome tegument and its surface membranes
The tegument surface of the adult schistosome, bounded by a normal plasma membrane overlain by a secreted membranocalyx, holds the key to understanding how schistosomes evade host immune responses. Recent advances in mass spectrometry (MS), and the sequencing of the Schistosoma mansoni transcriptome/genome, have facilitated schistosome proteomics. We detache
Memórias do Instituto Oswaldo Cruz. Publicado em: 2006-10
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4. Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice
Proteomic approaches require simple and efficient protein purification methodologies that are amenable to high throughput. Biotinylation is an attractive approach for protein complex purification due to the very high affinity of avidin/streptavidin for biotinylated templates. Here, we describe an approach for the single-step purification of transcription
National Academy of Sciences.
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5. A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli.
The high affinity binding interaction of biotin to avidin or streptavidin has been used widely in biochemistry and molecular biology, often in sensitive protein detection or protein capture applications. However, in vitro chemical techniques for protein biotinylation are not always successful, with some common problems being a lack of reaction specificity, i
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6. Repression of Transposable Elements by Histone Biotinylation12
Transposable elements constitute >40% of the human genome; transposition of these elements increases genome instability and cancer risk. Epigenetic mechanisms are important for transcriptional repression of retrotransposons, thereby preventing transposition events. Binding of biotin to histones, mediated by holocarboxylase synthetase (HCS), is a novel histon
American Society for Nutrition.
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7. Biotinylation of Bacillus thuringiensis Insecticidal Crystal Proteins
Biotinylation of Bacillus thuringiensis insecticidal crystal proteins (ICPs) was evaluated for its potential use in an alternative ICP screening method and in the characterization of ICP receptors. In vivo biological activity of CryIA(b), as inferred from bioassays with Manduca sexta and Ostrinia nubilalis and from histopathological effects on O. nubilalis m
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8. Regulation of [beta]-Methylcrotonyl-Coenzyme A Carboxylase Activity by Biotinylation of the Apoenzyme.
Regulation of the expression of the gene(s) coding for the 78-kD, biotin-containing subunit of [beta]-methylcrotonyl-coenzyme A carboxylase (MCCase) was investigated in different organs of tomato (Lycopersicon esculantus) plants. The specific activity of MCCase is highest in extracts from roots, followed in descending order by ripe and ripening fruits, stems
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9. Biotinylation in vivo as a sensitive indicator of protein secretion and membrane protein insertion.
Escherichia coli biotin ligase is a cytoplasmic protein which specifically biotinylates the biotin-accepting domains from a variety of organisms. This in vivo biotinylation can be used as a sensitive signal to study protein secretion and membrane protein insertion. When the biotin-accepting domain from the 1.3S subunit of Propionibacterium shermanii transcar
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10. Fluoride-cleavable biotinylation phosphoramidite for 5′-end-labeling and affinity purification of synthetic oligonucleotides
A fluoride-cleavable phosphoramidite for biotinylation was designed, synthesized and coupled efficiently to the 5′-end of DNA on an automatic synthesizer. The diisopropylsilyl acetal functionality was used to link the biotin moiety through a tertiary hydroxide group to the 5′-end of DNA. This linkage proved to be completely stable under certain post-synt
Oxford University Press.
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11. Biotinylation of Bacillus thuringiensis Insecticidal Crystal Proteins
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12. Melting studies of dangling-ended DNA hairpins: effects of end length, loop sequence and biotinylation of loop bases
The effects of 3′ single-strand dangling-ends of different lengths, sequence identity of hairpin loop, and hairpin loop biotinylation at different loop residues on DNA hairpin thermodynamic stability were investigated. Hairpins contained 16 bp stem regions and five base loops formed from the sequence, 5′-TAGTCGACGTGGTCC-N5-GGACCACGTCGACTAG-En-3′. The l
Oxford University Press.